abstract：AIM:Gonorrhoea remains a leading public health burden and the development of vaccine against gonorrhoea becomes more urgent. Here, a novel Neisseria gonorrhoeae DNA vaccine delivered by Salmonella enteritidis ghosts was developed and the immune responses of the vaccine candidate were evaluated. METHODS AND RESULTS:Nei...

journal_title：Journal of applied microbiology

authors： Jiao H,Yang H,Zheng W,Zhang Q,Zhao D,Li G

更新日期：2020-08-08 00:00:00

abstract：AIMS:The aim of this study was to develop a novel selective agar for the specific isolation and detection of Bacillus anthracis. METHODS AND RESULTS:Based on published data on antibiotic resistance and susceptibility of B. anthracis and other closely related species of the Bacillus cereus sensu lato group, a new selec...

journal_title：Journal of applied microbiology

authors： Rohde A,Papp S,Feige P,Grunow R,Kaspari O

更新日期：2020-08-01 00:00:00

abstract：AIMS:The study established the inactivation kinetic parameters of an Acanthamoeba cyst isolate subjected to heating and chlorination. METHODS AND RESULTS:A strain of Acanthamoeba was isolated and purified from an area surrounding a pilot food plant. Mature cysts (14 days) were subjected to heat inactivation studies at...

journal_title：Journal of applied microbiology

authors： Gabriel AA,Panaligan DC

更新日期：2020-08-01 00:00:00

abstract：AIM:To screen a lactic acid bacteria (LAB) strain for good adaption in alfalfa ensiling and to evaluate its effects on alfalfa ensiling. METHODS AND RESULTS:Lactobacillus plantarum (LP) strain ZZU203 was selected due to its superior ability to acidify structural carbohydrate metabolite and alfalfa powder. Alfalfa was ...

journal_title：Journal of applied microbiology

authors： Zhao SS,Wang YP,Yang FY,Wang Y,Zhang H

更新日期：2020-08-01 00:00:00

abstract：AIMS:To obtain the high-yield strain of fusidic acid, which is produced from fungus Fusidium coccineum and is the only fusidane-type antibiotic that has been used clinically, and confirm the changes in the transcription levels involved in increasing its production. METHODS AND RESULTS:By using the atmospheric and room...

journal_title：Journal of applied microbiology

authors： Huang WW,Ge XY,Huang Y,Chai XT,Zhang L,Zhang YX,Deng LN,Liu CQ,Xu H,Gao J

更新日期：2020-07-30 00:00:00

abstract：AIMS:Bacillus cereus sensu lato is a complex group of closely related bacteria, which are commonly present in the natural environment and food products. These organisms may cause food poisoning and spoilage as well as opportunistic infections. Thus far, their resistance to selected antibiotics has been explored only in...

journal_title：Journal of applied microbiology

authors： Bartoszewicz M,Czyżewska U

更新日期：2020-07-21 00:00:00

abstract：AIMS:To understand the genetics involved in surface attachment and biofilm formation of Listeria monocytogenes. METHODS AND RESULTS:An in vitro screen of a Himar1 transposon library of L. monocytogenes strain 15G01 identified three transposants that produced significantly different biofilm levels when compared to the ...

journal_title：Journal of applied microbiology

authors： Nowak J,Visnovsky SB,Cruz CD,Fletcher GC,van Vliet AHM,Hedderley D,Butler R,Flint S,Palmer J,Pitman AR

更新日期：2020-07-20 00:00:00

abstract：AIMS:The purpose of this study was to evaluate the ISO 16266 membrane filtration culture-based method for the detection of Pseudomonas aeruginosa from cooling tower and related water samples and to compare the performance of the Pseudalert MPN method (Pseudalert™/Quanti-Tray® ) for the enumeration of P. aeruginosa with...

journal_title：Journal of applied microbiology

authors： Spies K,Pleischl S,Sartory D

更新日期：2020-06-01 00:00:00

abstract：AIM:Identification and enumeration of foodborne pathogens in food stuffs are valuable concerns. In the present study, starch-blood-egg yolk-polymyxin B-trimethoprim-ceftazidime (SBYPTC) agar was established to isolate and specify the number of Bacillus cereus in food products. METHODS AND RESULTS:The effectiveness of ...

journal_title：Journal of applied microbiology

authors： Talahmeh N,Abu-Rumeileh S,Al-Razem F

更新日期：2020-05-01 00:00:00

abstract：AIMS:As cell-adapted foot-and-mouth disease virus (FMDV) with H56R mutation in VP3 has reduced thermostability, this study aimed to investigate the effect of thermostabilizers on cell-adapted FMDV for vaccine production. METHODS AND RESULTS:We examined the effect of 3% sucrose, 10% (or 25%) glycerol or 10% FBS on cell...

journal_title：Journal of applied microbiology

authors： Hwang JH,Moon Y,Lee G,Kim MY,Lee KN,Park JH,Lee M,Kim B,Kim SM

更新日期：2020-05-01 00:00:00

abstract：AIMS:To develop a method that is able to determine the microbial reduction in different dishwasher cleaning cycles and differentiate between different program parameters used. METHODS AND RESULTS:Stainless steel biomonitors were contaminated with Micrococcus luteus or Entereococcus faecium and cleaned in a specially p...

journal_title：Journal of applied microbiology

authors： Brands B,Schulze Struchtrup S,Stamminger R,Bockmühl DP

更新日期：2020-05-01 00:00:00

abstract：AIMS:To evaluate the inhibitory effect of five structurally different imidazolium salts on the in vitro growth of plant pathogenic bacteria that belong to divergent taxonomic genera as well as their ability to reduce the severity of common bacterial blight of common bean caused by Xanthomonas axonopodis pv. phaseoli an...

journal_title：Journal of applied microbiology

authors： Neves YF,Eloi ACL,de Freitas HMM,Soares EGO,Rivillo D,Demétrio da Silva V,Schrekker HS,Badel JL

更新日期：2020-05-01 00:00:00

abstract：AIMS:The purpose of this study was to evaluate the effects of altered environmental conditions on the persistence of Francisella tularensis bacteria and Venezuelan equine encephalitis virus (VEEV), on two material types. METHODS AND RESULTS:Francisella tularensis (F.t.) and VEEV were inoculated (c. 1 × 108 colony-form...

journal_title：Journal of applied microbiology

authors： Richter WR,Sunderman MM,Wendling MQS,Serre S,Mickelsen L,Rupert R,Wood J,Choi Y,Willenberg Z,Calfee MW

更新日期：2020-04-01 00:00:00

abstract：AIMS:To determine individual antibacterial and synergistic antibacterial effects of resveratrol and alcohol against Helicobacter pylori 26695 in vitro, and to elucidate the underlying mechanism of action of resveratrol against H. pylori. METHODS AND RESULTS:The minimum inhibitory concentrations (MICs) and time-killing...

journal_title：Journal of applied microbiology

authors： Xia M,Chen H,Liu S

更新日期：2020-04-01 00:00:00

abstract：AIMS:To extract and identify the metabolites of strain A217 as well as its antifungal spectrum and control effect on various plant pathogens. METHODS AND RESULTS:Strain A217 was identified as a Streptomyces sp. which was most similar to Streptomyces lienomycini. An antimicrobial spectrum test indicated that strain A21...

journal_title：Journal of applied microbiology

authors： He H,Hao X,Zhou W,Shi N,Feng J,Han L

更新日期：2020-04-01 00:00:00

abstract：AIM:The aim of the present study was to investigate the in vitro antioxidant activity of yeast strains isolated from virgin olive oil. METHODS AND RESULTS:The 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free-radical scavenging activity of single cultures of 24 yeast strains belonging to eight species isolated from virgin ol...

journal_title：Journal of applied microbiology

authors： Ciafardini G,Zullo BA

更新日期：2020-03-01 00:00:00

abstract：AIMS:To study the individual and combined contribution of catechin, protocatechuic and vanillic acids to inhibit the adhesion of uropathogenic Escherichia coli (UPEC) on the surface of silicone catheters. METHODS AND RESULTS:The adhesion of UPEC to silicone catheters during the exposure to nonlethal concentrations of ...

journal_title：Journal of applied microbiology

authors： Bernal-Mercado AT,Gutierrez-Pacheco MM,Encinas-Basurto D,Mata-Haro V,Lopez-Zavala AA,Islas-Osuna MA,Gonzalez-Aguilar GA,Ayala-Zavala JF

更新日期：2020-02-01 00:00:00

abstract：AIMS:Probiotics have the ability to enhance the immune system, produce anti-inflammatory action and promote wound healing process. The first aim of the study was to isolate pathogenic micro-organisms from sites of chronic ulcerative lesion. The second aim was to evaluate probiotic efficacy of SYNBIO® (1:1 combination o...

journal_title：Journal of applied microbiology

authors： Coman MM,Mazzotti L,Silvi S,Scalise A,Orpianesi C,Cresci A,Verdenelli MC

更新日期：2020-02-01 00:00:00

abstract：AIMS:To add a spore germination step in order to reduce decontamination temperature and time requirements compared to the current hot, humid air decontamination parameters, which are 75-80°C, ≥72 h, 70-90% RH, down to ≤60°C and ≤24 h total decontamination time. METHODS AND RESULTS:Bacillus anthracis spore germination ...

journal_title：Journal of applied microbiology

authors： Buhr TL,Minter ZA,Kennihan NL,Young AA,Borgers-Klonkowski EL,Osborn EB,Bohmke MD,Hamilton SM,Kimani MB,Miller CT,Mackie RS,Innocenti JM,Bensman MD,Lilly SD

更新日期：2020-01-01 00:00:00

abstract：AIMS:To determine the composition and temporal stability of the gut (faecal) microbiota of sheep (Ovis aries). METHODS AND RESULTS:Microbial population dynamics was conducted using ARISA (28 sheep) and 16S rRNA sequencing (11 sheep). Firmicutes and Bacteroidetes were the predominant bacterial phyla, constituting ~80% ...

journal_title：Journal of applied microbiology

authors： Mamun MAA,Sandeman M,Rayment P,Brook-Carter P,Scholes E,Kasinadhuni N,Piedrafita D,Greenhill AR

更新日期：2020-01-01 00:00:00

abstract：AIMS:To determine the immune gene expression response of gilthead seabream (Sparus aurata) that is experimentally infected with the lymphocystivirus LCDV-Sa. METHODS AND RESULTS:Viral DNA and transcripts were detected by qPCR in all samples from fish injected with LCDV-Sa, demonstrating that the virus establish a syst...

journal_title：Journal of applied microbiology

authors： Leiva-Rebollo R,Labella AM,Borrego JJ,Castro D

更新日期：2020-01-01 00:00:00

abstract：AIMS:To investigate the genetic determinates for conjugated linolenic acid (CLNA) production in Lactobacillus plantarum ZS2058, a high CLNA producer. METHODS AND RESULTS:After culturing with α-linolenic acid (ALA) in the medium, the fatty acid compositions of supernatant fluid and cell pellets were analysed via GC-MS....

journal_title：Journal of applied microbiology

authors： Yang B,Chen H,Gao H,Ren Q,Zhang H,Chen W

更新日期：2020-01-01 00:00:00

abstract：AIMS:The outer membrane porin protein (OMPP) of Bordetella bronchiseptica is an important adhesion factor and protective immunogen. The aim of this study was to verify the immunogenicity of recombinant OMPP and its protective efficacy against a lethal challenge with B. bronchiseptica in rabbits. METHODS AND RESULTS:So...

journal_title：Journal of applied microbiology

authors： Zhang H,Zhang H,Xiong B,Fan G,Cao Z

更新日期：2019-12-01 00:00:00

abstract：AIMS:This study aims to evaluate the impact of solid-state fermentation (SSF) by Trichoderma reesei on the phenolic content, antioxidant and antimicrobial activities of garden cress seeds (GCS). METHODS AND RESULTS:The factorial statistical design was employed to optimize the SSF conditions, incubation time, pH, tempe...

journal_title：Journal of applied microbiology

authors： Abdel-Aty AM,Bassuiny RI,Barakat AZ,Mohamed SA

更新日期：2019-11-01 00:00:00

abstract：AIMS:This study was conducted to evaluate the chemical composition and in vitro gas production (GP) and fermentation parameters of Quercus infectoria and Quercus libani leaves following treatment with the Klebsiella pneumoniae, a tannin-degrading bacterium. METHODS AND RESULTS:This isolate was isolated on medium conta...

journal_title：Journal of applied microbiology

authors： Motamedi H,Azizi A,Ahmadi M

更新日期：2019-11-01 00:00:00

abstract：AIMS:To investigate the effects of ohmic heating (OH) and water bath heating (WB) on the membrane permeability, membrane structure, intracellular organization and leakage of intracellular substances of Escherichia coli O157:H7 at the same inactivation level and at a heating temperature of 72°C. METHODS AND RESULTS:Flo...

journal_title：Journal of applied microbiology

authors： Tian X,Shao L,Yu Q,Liu Y,Li X,Dai R

更新日期：2019-11-01 00:00:00

abstract：AIM:To evaluate the activity and effectiveness of impregnated central venous catheters (CVC) against Klebsiella pneumoniae biofilms. METHODS AND RESULTS:The antimicrobial activity and durability of impregnated-CVCs were evaluated over time and the size of zones of inhibition (ZI) was measured. Biofilm formation was ob...

journal_title：Journal of applied microbiology

authors： de Sousa JKT,Haddad JPA,de Oliveira AC,Vieira CD,Dos Santos SG

更新日期：2019-10-01 00:00:00

abstract：AIMS:The purposes were to evaluate the detection of low levels of Campylobacter in water by dead-end ultrafiltration (DEUF) to determine the sensitivity and suitability for use under field condition. METHODS AND RESULTS:The DEUF technique followed by detection according to ISO 10272 was tested on artificially and natu...

journal_title：Journal of applied microbiology

authors： Ferrari S,Frosth S,Svensson L,Fernström LL,Skarin H,Hansson I

更新日期：2019-10-01 00:00:00

abstract：AIMS:To evaluate efficacy of Saccharomyces cerevisiae fermentate prebiotic (EH) in protection of intestinal barrier integrity in rats during heat stress, to analyze the impact of heat stress and preventive treatment with EH on the structure of the gut microbiota. METHODS AND RESULTS:Two groups of rats were treated ora...

journal_title：Journal of applied microbiology

authors： Ducray HAG,Globa L,Pustovyy O,Morrison E,Vodyanoy V,Sorokulova I

更新日期：2019-10-01 00:00:00

abstract：AIMS:The formation of metabolically inactive and nongrowing cells is an inevitable by-product of intensive fermentation. This study investigated whether co-feeding can be used to resuscitate nongrowing Acetobacter senegalensis cells to enable them to produce gluconic acid in successive fermentation runs at 38°C. METHO...

journal_title：Journal of applied microbiology

authors： Zarmehrkhorshid R,Shafiei R,Delvigne F

更新日期：2019-10-01 00:00:00

abstract：AIMS:The aim was to isolate, identify and characterize yeasts present in rumen fluid and to select strains showing potential as probiotics. METHODS AND RESULTS:Rumen fluid was sampled from 4 herds of dairy and beef cattle and 77 yeast isolates were identified. Initial screening was based on the capacity to maintain vi...

journal_title：Journal of applied microbiology

authors： Fernandes T,Carvalho BF,Mantovani HC,Schwan RF,Ávila CLS

更新日期：2019-09-01 00:00:00

abstract：AIMS:The objective of this study was to compare qualitatively and quantitatively the results of identification of the bacteria present in milk samples from cows with subclinical mastitis using multiplex qPCR assay and matrix-assisted laser desorption-ionization time of flight mass spectrometry (MALDI-TOF MS® ) after ba...

journal_title：Journal of applied microbiology

authors： Ngassam Tchamba C,Rao AS,Boyen F,Haesebrouck F,Duprez JN,Théron L,Thiry D,Mainil JG

更新日期：2019-09-01 00:00:00

abstract：AIM:Assess performance of broth microdilution (BMD) as well as agar dilution methods for antimicrobial susceptibility testing of Staphylococci using tetrazolium salt. METHODS AND RESULTS:Minimum inhibitory concentration (MIC) of eight antimicrobials; vancomycin (VA), linezolid, oxacillin, gentamicin (CN), tetracycline...

journal_title：Journal of applied microbiology

authors： Mahmoud BS,ElMasry SA,Fahim NAEMM,Abd ElSattar MA,Shaker OA

更新日期：2019-09-01 00:00:00

abstract：AIM:Shiga toxin-producing Escherichia coli (STEC) cause bloody diarrhoea, kidney failure and occasionally death. However, identifying the source of infection caused by STEC other than serogroup O157 is hampered by the availability of sensitive methods for detecting these pathogens. In this study, we developed novel too...

journal_title：Journal of applied microbiology

authors： Kirchner M,Sayers E,Cawthraw S,Duggett N,Gosling R,Jenkins C,Dallman TJ,Mueller-Doblies D,Anjum MF

更新日期：2019-09-01 00:00:00

abstract：AIMS:This study evaluated methods to sample and extract nucleic acids from Pacific oysters to accurately determine the microbiome associated with different tissues. METHODS AND RESULTS:Samples were collected from haemolymph, gill, gut and adductor muscle, using swabs and homogenates of solid tissues. Nucleic acids wer...

journal_title：Journal of applied microbiology

authors： Pathirana E,McPherson A,Whittington R,Hick P

更新日期：2019-08-01 00:00:00

abstract：AIMS:The current study aimed to assess the potential of a new high dose ultraviolet (UV) disinfection device to inactivate methicillin-resistant Staphylococcus aureus (MRSA), Clostridium difficile and a norovirus surrogate on handheld mobile devices, and to compare the efficacy of the UV-C device to hydrogen peroxide d...

journal_title：Journal of applied microbiology

authors： Wallace RL,Ouellette M,Jean J

更新日期：2019-08-01 00:00:00

abstract：AIMS:One of the major limitations to the production of alfalfa (Medicago sativa) is the fungus Phoma medicaginis, which infects alfalfa and causes leaf spots. This study aims to understand alfalfa's response to P. medicaginis infection, the colonization of arbuscular mycorrhizal fungus (AMF) and the effect of AMF on pl...

journal_title：Journal of applied microbiology

authors： Li Y,Duan T,Nan Z,Li Y

更新日期：2019-07-16 00:00:00

abstract：:Besides the liver, rumen is one of the most important components of metabolism in ruminants. However, the microbes residing in the rumen are influenced by several complex factors such as diet, which result in fluctuations in the rumen pH. Rumen pH affects feed intake and feed digestibility, subsequently causing microb...

journal_title：Journal of applied microbiology

authors： Faniyi TO,Adegbeye MJ,Elghandour MMMY,Pilego AB,Salem AZM,Olaniyi TA,Adediran O,Adewumi MK

更新日期：2019-07-01 00:00:00

abstract：AIMS:To evaluate ATP bioluminescence-based hygiene monitoring systems under conditions relevant to fish processing environments. METHODS AND RESULTS:The ATP bioluminescence of fish fractions that are potentially present after insufficient cleaning of fish processing environments was determined. Different fractions and...

journal_title：Journal of applied microbiology

authors： Møretrø T,Normann MA,Saebø HR,Langsrud S

更新日期：2019-07-01 00:00:00

abstract：AIMS:The aim of this study was to evaluate the persistence of Pseudomonas fluorescens LBUM677 in the rhizosphere of Buglossoides arvensis under agricultural field conditions and determine if B. arvensis intraspecific genetic variations affect the capacity of LBUM677 to colonize its rhizosphere and increase seed oil and...

journal_title：Journal of applied microbiology

authors： Novinscak A,Filion M

更新日期：2019-07-01 00:00:00